(Na~++K~+)—ATP酶与磷脂膜的相互作用 |
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引用本文: | 王平原,黄芬. (Na~++K~+)—ATP酶与磷脂膜的相互作用[J]. 生物物理学报, 1991, 7(2): 217-222 |
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作者姓名: | 王平原 黄芬 |
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作者单位: | 中国科学院生物物理研究所,北京100080 |
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摘 要: | 本文研究了不同磷脂对兔肾外髓质(Na~++K~+)-ATP酶活性的影响、结果表明,DOPC、PG重组活性最高,用DMPC重组导致酶失活,酸性磷脂有利于维持该酶活性.DSC及自旋标记ESR实验结果示出(Na~++K~+)-ATP酶有选择地与酸性磷脂相互作用.
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关 键 词: | 磷脂膜 ATP酶 |
INTERACTION OF (Na~+ + K~+) -ATPase WITH PHOSPHOLIPID LIPOSOMES |
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Abstract: | The effect of different kinds of phospholipid on the activity of the (Na+ + K+) - ATPase from rabbit Kidney outer medulla have been investigated. The highest activity was observed when the enzyme reconstituted with dioleoylphosphotidylcho-line (DOPC) and phosphotidylglycerol (PG), whereas enzyme reconstituted with di-myristoylphosphotidyl choline(DMPC) leads to complete inactivalion. The enzyme has been found to be activated optimally by acid phospholipids. DSC and spin label ESR experiments also demonstrated that the (Na+ + K+) - ATPase displays a selectivity for certain acid phospholipids. |
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