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| 产1,3-丙二醇基因工程菌发酵条件的研究 | |
| 引用本文： | 韦旭钦,陈发忠,罗兆飞,韦宇拓,周文广,黄日波. 产1,3-丙二醇基因工程菌发酵条件的研究[J]. 生物加工过程, 2006, 4(1): 39-43 |
| 作者姓名： | 韦旭钦 陈发忠 罗兆飞 韦宇拓 周文广 黄日波 |
| 作者单位： | 1. 广西大学,生命科学与技术学院,发酵与酶工程研究所,南宁,530005;广西南宁中诺生物工程有限责任公司,南宁,530003 2. 广西南宁中诺生物工程有限责任公司,南宁,530003 3. 广西大学,生命科学与技术学院,发酵与酶工程研究所,南宁,530005 |
| 基金项目： | 国家“863”高科技资助项目(2003AA001039) |
| 摘 要： | 利用途径工程的方法,将来源于克雷伯氏菌(Klebsiella pneumoniae)的甘油脱水酶基因dhaB和1,3-丙二醇氧化还原酶基因dhaT构建成多顺反子重组质粒pSE-dhaB-dhaT并在大肠杆菌JM 109中进行表达,在大肠杆菌中构建一条新的产1,3-丙二醇代谢途径。研究表明,重组菌株JM 109/pSE-dhaB-dhaT在微好氧条件下,尝试用廉价的乳糖为诱导物、维生素B12为辅酶,可以将甘油转化为1,3-丙二醇,产量达15.34 g/L,甘油转化率为35.7%,对低成本生产1,3-丙二醇作了有益的探索。 |
| 关 键 词： | 1 3-丙二醇 重组菌 发酵条件 |
| 文章编号： | 1672-3678（2006）01-0039-05 |
| 收稿时间： | 2006-01-10 |
| 修稿时间： | 2006-01-10 |
Study of the fermentation conditions for the recombinant microorganism producing 1,3-propanediol |
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| WEI Xu-qin,CHEN Fa-zhong,LUO Zhao-fei,WEI Yu-tuo,ZHOU Wen-guang,HUANG Ri-bo. Study of the fermentation conditions for the recombinant microorganism producing 1,3-propanediol[J]. Chinese Journal of Bioprocess Engineering, 2006, 4(1): 39-43 | |
| Authors: | WEI Xu-qin CHEN Fa-zhong LUO Zhao-fei WEI Yu-tuo ZHOU Wen-guang HUANG Ri-bo |
| Affiliation: | 1. Institute of Fermentation and Enzyme engineering GuangXi University, Nanning 530005, China; 2. Sinozyme Biotech Co. Ltd Keyuan Av, Nanning 530004, China |
| Abstract: | Based on the principle of the pathway engineering,a novel pathway of producing 1,3-propanediol was built in E.coli.The dhaB gene encoding glycerol dehydratase(DHAB) and the dhaT gene encoding 1,3-propanediol oxidoreductase(DHAT) were cloned from Klebsiella pneumoniae and then inserted into expression vector pSE380 to obtain recombinant polycistron plasmid pSE-dhaB-dhaT.This polycistron was transformed into E.coli JM109.The result showed that the recombinant microorganism JM109/pSE-dhaBdhaT could convert glycerol to 1,3-propanediol directly under the induced conditions of lactose and vitamin B_(12) in the fermentor,and the maximal concentration of 1,3-propanediol was 15.34 g/L at 60 h,conversion rate of glycerol was 35.7% under the microaerobic condition. |
| Keywords: | 1 3-propanediol recombinant microorganism ferment conditions |
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