Leishmania mexicana amazonensis: plasma membrane adenine nucleotide translocator and chemotaxis |
| |
Authors: | Detke S Elsabrouty R |
| |
Affiliation: | Department of Biochemistry and Molecular Biology, School of Medicine and Health Sciences, University of North Dakota, 501 N. Columbia Road, Grand Forks, ND 58203, USA. sdetke@medicine.nodak.edu |
| |
Abstract: | Leishmania cannot synthesize purines de novo and rely on their host to furnish these compounds. To accomplish this, they possess multiple purine nucleoside and nucleobase transporters. Subcellular fractionation, immunohistochemical localization with anti-adenine nucleotide translocator (ANT) antibodies and surface biotinylation show that the mitochondrial ANT is also present in the plasma membrane of both promastigotes and amastigotes. Leishmania, however, do not appear to rely on this transporter to supplement their purine or energy requirements via preformed ATP from its host. Rather, Leishmania appear to use the plasma membrane ANT as part of a chemotaxis response. ATP is a chemorepellant for Leishmania and cells treated with atractyloside, an inhibitor of ANT, no longer exhibit negative chemotaxis for this compound. |
| |
Keywords: | Leishmania TOR Mitochondria Kinetoplast Plasma membrane Chemotaxis Adenine nucleotide translocator AKII, adenylate kinase II ANT, adenine nucleotide translocator CPT, carnitine palmitoyl transferase GFP, green fluorescent protein LPG, lipophosphoglycan MPTP, mitochondrial permeability transition pore complex YFP, yellow fluorescent protein |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|