Partition behaviour of horseradish peroxidase isoenzymes in aqueous two-phase poly(ethyleneglycol)/phosphate systems |
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Authors: | María V Miranda Osvaldo Cascone |
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Institution: | (1) Cátedra de Microbiología Industrial y Biotecnología. Facultad de Farmacia y Bioquímica, Junín 956, (1113) Buenos Aires, Argentina |
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Abstract: | Summary Partition of purified horseradish peroxidase isoenzymes in aqueous two-phase systems was not affected by pH changes, but tie-line length and NaCl addition greatly increased the partition coefficient of all three isoenzymes, the former having more influence than the latter. In all systems, K were higher for acidic than those for neutral and basic isoenzymes, and K for basic were the lowest. |
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