Charge effect in the interaction of doxorubicin and derivatives with polydeoxynucleotides. |
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Authors: | J Ruggiero L E Xodo A Ciana G Manzini F Quadrifoglio |
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Affiliation: | Physics Department, Institute of Biosciences, UNESP, S?o José do Rio Preto, Brazil. |
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Abstract: | The equilibrium interaction of doxorubicin and its N-acetyl derivative with a series of purine-pyrimidine alternating polydeoxynucleotides has been studied through spectrofluorometry to assess the relevance of the electrostatic contribution to DNA intercalation. The results have shown that: (a) the suppression of the positive charge on the aminosugar has: (I) a profound negative effect on the free energy of intercalation, as expected, and (II) a negligible influence on the base specificity, which supports the notion of an essentially electrostatic effect of N-acetylation on intercalation; (b) a reasonably good accord with the demands of a polyelectrolytic model, due to Friedman and Manning, is found. |
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