Ca2+ Current-Deficient Pawn Mutants are Promoted to Queens During Chronic Depolarization of Paramecium tetraurelia |
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Authors: | RR Preston JA Hammond |
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Institution: | (1) Department of Pharmacology and Physiology, MCP Hahnemann University, 2900 Queen Lane, Philadelphia, PA 19129, USA, US |
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Abstract: | Chronic KCl-induced depolarization of Paramecium tetraurelia enhances Ca2+-dependent backward swimming behavior over a period of 8–24 hr. Here, we investigated the electrophysiological mechanisms
underlying this adaptive phenomenon using voltage-clamp techniques. Cells that had been adapted to 20 mm KCl showed several significant changes in the properties of the Ca2+ current that mediates ciliary reversal in Paramecium (I
Ca
), including a positive shift in voltage sensitivity and a significant slowing of inactivation. In seeking an explanation
for these changes, we examined the effects of chronic depolarization on mutants that do not normally express a Ca2+ current or swim backward. Surprisingly, pawn B mutant cells slowly regained the ability to reverse their cilia during KCl exposure with a time course that mirrored behavioral
adaptation of the wild type. This behavior was accompanied by expression of a novel Ca2+ current (I
QUEEN
) whose voltage sensitivity was shifted positive with respect to the wild-type Ca2+ current and that was slow to inactivate. Coincidental expression of I
QUEEN
in the wild type during adaptation would readily explain the observed changes in I
Ca
kinetics. We also examined the effects of chronic depolarization on Dancer, a mutant suggested previously to have an I
Ca
inactivation defect. The mutant phenotype could be suppressed or exaggerated greatly by manipulating extracellular KCl concentration,
suggesting that Dancer lesion instead causes inappropriate regulation of I
QUEEN
.
Received: 23 April 1999/Revised: 29 June 1999 |
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Keywords: | :Paramecium— Ca2+ current — Mutation — Adaptation — Depolarization — Behavior |
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