pH-dependent quenching of the fluorescence of tryptophan residues in class A beta-lactamase from E. coli (TEM-1) |
| |
| Authors: | Christov Christo Ianev Dimitar Shosheva Alexandra Atanasov Boris |
| |
| Affiliation: | Institute of Organic Chemistry Bulgarian Academy of Science, Sofia 1113, Bulgaria. christov@sg.uji.es |
| |
| Abstract: | We performed an investigation of the pH-dependent quenching of the fluorescence of tryptophan residues of TEM-1 beta-lactamase from E. coli by uncharged and charged quenchers. pH-dependent Stern-Volmer constants (Ksv/pH) of tryptophan residues allowed us to determine subtle but discrete structurally and functionally important processes. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
