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Effect of age and type of tissue on genetic transformation of Lotus corniculatus by Agrobacterium tumefaciens
Authors:Ian P Armstead  K Judith Webb
Institution:(1) Welsh Plant Breeding Station, Plas Gogerddan, SY23 3EB Aberystwyth, Dyfed, UK;(2) Present address: Plant Breeding Institute, Maris Lane, CB2 2LQ Trumpington, Cambridge, UK
Abstract:Various experiments of Lotus corniculatus cv. Leo were infected with Agrobacterium tumefaciens strains C58 (wild-type) and GV3101 (control). A maximum of 83 per cent of cotyledons excised from 7 day old seedlings and 63 per cent of leaves excised from seedlings grown in vitro formed galls in culture. The genotype of the seedling had an effect on the response.
Keywords:Agrobacterium tumefaciens  herbage legumes  genetic transformation
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