Effect of age and type of tissue on genetic transformation of Lotus corniculatus by Agrobacterium tumefaciens |
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Authors: | Ian P Armstead K Judith Webb |
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Institution: | (1) Welsh Plant Breeding Station, Plas Gogerddan, SY23 3EB Aberystwyth, Dyfed, UK;(2) Present address: Plant Breeding Institute, Maris Lane, CB2 2LQ Trumpington, Cambridge, UK |
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Abstract: | Various experiments of Lotus corniculatus cv. Leo were infected with Agrobacterium tumefaciens strains C58 (wild-type) and GV3101 (control). A maximum of 83 per cent of cotyledons excised from 7 day old seedlings and 63 per cent of leaves excised from seedlings grown in vitro formed galls in culture. The genotype of the seedling had an effect on the response. |
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Keywords: | Agrobacterium tumefaciens herbage legumes genetic transformation |
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