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| 四株抗荔枝病原菌的芽孢杆菌的分离鉴定 | |
| 引用本文： | 黄庶识,黄曦,许兰兰,张荣灿,许铭本,黄荣韶. 四株抗荔枝病原菌的芽孢杆菌的分离鉴定[J]. 生物技术通报, 2010, 0(12) |
| 作者姓名： | 黄庶识 黄曦 许兰兰 张荣灿 许铭本 黄荣韶 |
| 作者单位： | 1. 广西科学院生物物理实验室,南宁,530007;昆明理工大学生物工程技术研究中心,昆明,650224 2. 广西科学院生物物理实验室,南宁,530007;广西大学农学院,南宁,530007 3. 广西科学院海洋环境监测中心,南宁,530007 4. 广西大学农学院,南宁,530007 |
| 基金项目： | 国家自然科学基金项目(30860010); 广西科学基金项目(桂科回0832006); 广西科研院所基本科研业务费资助项目(08YJ16WL01) |
| 摘 要： | 荔枝果树根际土壤中筛选出4株芽孢杆菌OR-1、OR-2、OR-3、ON-6,都显示出抗荔枝病原菌的活性。采取对该菌株形态特征、培养特征、生理生化特征和遗传特性进行研究的方法,结果表明菌株与枯草芽孢杆菌(Bacillus subtilis)的特征一致;将4菌株的16S rDNA序列在GenBank中进行序列比对,结果亦显示其与Bacillus subtilis的16S rDNA的序列片段的相似性均达99%以上;以相似性为基础构建系统进化树,分析表明菌株与Bacillus subtilis同源关系最近。最终得出结论为菌株OR-1、OR-2、OR-3、ON-6为枯草芽孢杆菌。 |
| 关 键 词： | 枯草芽孢杆菌 荔枝病原菌 分离鉴定 16S rDNA测序 |
Isolation and Identification of Four Bacillus Strains with Antagonistic Ability Against Litchi Pathogenic Fungi |
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| Huang Shushi,Huang Xi,Xu Lanlan,Zhang Rongcan,Xu Mingben,Huang Rongshao. Isolation and Identification of Four Bacillus Strains with Antagonistic Ability Against Litchi Pathogenic Fungi[J]. Biotechnology Bulletin, 2010, 0(12) | |
| Authors: | Huang Shushi Huang Xi Xu Lanlan Zhang Rongcan Xu Mingben Huang Rongshao |
| Affiliation: | Huang Shushi1,3 Huang Xi1,2 Xu Lanlan1,3 Zhang Rongcan4Xu Mingben4 Huang Rongshao2(1Biophysics Laboratory,Guangxi Academy of Sciences,Nanning 530007,2Agriculture Academy of Guangxi University,3Biotechnology Research Center,Kunming University of Science and Technology,Kunming 650224,4Marine Enviroment Monitoring Center,Nanning 530007) |
| Abstract: | Four strains of Bacillus OR-1,OR-2,OR-3,ON-6,were isolated from rhizosphere soil of litchi,all of which showed the inhibitory activity of against litchi pathogens fungi.The investigation of their formal,cultural and physiological characteristics indicated that they had the same characteristic with Bacillus subtilis.Comparis on of their 16S rDNA sequence with the data in GenBank,showed the similarity of 99% or more between the four strains and Bacillus subtilis.Phylogenetic tree based on the similarity was e... |
| Keywords: | Bacillus subtilis Litchi pathogens fungi Isolation and identification Analyzing 16S rDNA sequence |
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