Non-chromogenic peptide substrates for detection of enzymes by means of capacitance changes at metal electrodes |
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| Authors: | H Arwin E Agner I Lundstr?m G Claeson |
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| Institution: | Department of Physics and Measurement Technology, Link?ping Institute of Technology, Sweden. |
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| Abstract: | A new type of substrate for enzyme detection has been developed. The substrate is non-chromogenic and is used in an assay method based on electrode adsorption. The rate of change in the electric capacitance of the electrode is monitored and taken as a measure of the substrate adsorption. Substrate adsorption is in turn proportional to substrate bulk concentration and thus subject to changes by enzymes. The new substrate introduces a new concept in enzyme detection: as it is non-chromogenic it may contain appropriate amino acids on both sides of the bond subject to enzymatic cleavage. |
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