Protein A insensitive ELISA detection of staphylococcal enterotoxin B |
| |
| Authors: | V Rogemond JM Da Costa Castro T Delaunay C Cazaux RMF Guinet † |
| |
| Institution: | Centre d'Immunochimie Microbienne, Institut Pasteur de Lyon, L'Arbresle, France. |
| |
| Abstract: | A sandwich enzyme-linked immunosorbent assay to detect staphylococcal enterotoxin B was developed using rat monoclonal antibodies as capture antibodies and as a biotinylated conjugate. This test was sensitive, less than 1 ng/ml of enterotoxin B was detected and interference by protein A was prevented by the use of rat monoclonal antibodies of the IgG2a isotype which were insensitive to protein A even at concentrations greater than 1000 ng/ml. |
| |
| Keywords: | |
|
|
