Crystal structure of yeast phenylalanine transfer RNA. II. Structural features and functional implications. |
| |
Authors: | S R Holbrook J L Sussman R W Warrant S H Kim |
| |
Affiliation: | Department of Biochemistry Duke University Medical Center Durham, N.C. 27710, U.S.A. |
| |
Abstract: | The structural features of yeast phenylalanine transfer RNA are analyzed and documented in detail, based on atomic co-ordinates obtained from an extensive crystallographic refinement of the crystal structure of the molecule at 2.7 Å resolution (see preceding paper). We describe here: the relative orientation and the helicity of the base-paired stems; more definitive assignments of tertiary hydrogen bonds involving bases, riboses and phosphates; binding sites for magnesium hydrates, spermine and water; iriter-molecular contacts and base-stacking; flexibility of the molecule; conformational analysis of nucleotides in the structure. Among the more noteworthy features are a considerable irregularity in the helicity of the base-paired stems, a greater flexibility in the anticodon and aminoacyl acceptor arms, and a “coupling” among several conformational angles. The functional implications of these structural features are also discussed. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|