Association of nucleosidediphosphate kinase with microtubules. |
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Authors: | J A Nickerson W W Wells |
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Institution: | Department of Biochemistry, Michigan State University East Lansing, Michigan 48824 USA |
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Abstract: | A nucleosidediphosphate kinase activity (EC 2.7.4.6) which phosphorylates GDP to GTP is present in bovine brain microtubule protein prepared by cycles of assembly-disassembly. This activity persists through 5 cycles of assembly-disassembly and sediments with microtubules in sucrose density gradients, but is not associated with the tubulin dimer. It is proposed that the kinase is an integral part of the microtubule and is therefore a microtubule associated protein (MAP). Several isozymes of nucleosidediphosphate kinase exist in our preparations with a pI 7.6 form predominant. It may be speculated that this enzyme affects tubulin assembly by modulating the ratio in the microtubule environment. |
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Keywords: | To whom inquiries should be addressed |
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