脂溶性维生素A、D、E与妊娠期糖尿病的相关性分析

目的:探讨妊娠期糖尿病(gestational diabetes mellitus,GDM)孕妇血清中维生素A、25-羟基维生素D、维生素E的水平及其临床意义。方法:选取在大连大学附属中山医院就诊并诊断为GDM的孕妇1000例为实验组;另选取无GDM孕妇1000例为对照组。采用反向高频液相色谱荧光法(high-performance liquid chromatography,HPLC)测定其血清维生素A、E水平;高效液相色谱液相色谱串联质谱法(high-performance liquid chromatography tandem mass spectrometer,HPLC-AS/MS)测定血清25-羟基维生素D水平。结果:GDM孕妇血清中维生素A水平与无GDM孕妇比较差异无统计学意义(P>0.05);GDM孕妇血清25-羟基维生素D水平明显低于对照组(P<0.05);GDM孕妇血清维生素E水平明显高于对照组(P<0.05)。GDM孕妇血清25-羟基维生素D水平与血清维生素E水平呈显著负相关(r=-0.351,P<0.05)。结论:GDM孕妇血清25-羟基维生素D水平低,维生素E水平高,且两者有一定相关性,可能共同参与了GDM的发生。

Correlative analysis between Fat-soluble vitamins A, D and E and Gestational Diabetes

ABSTRACT Objective: To investigate the expression levels and clinical significance of vitamin A, 25-hydroxyvitamin D, vitamin E in pregnant women with gestational diabetes mellitus. Methods: One hundred patients with GDM diagnosed in Zhongshan Hospital Affiliated to Dalian University were selected as experimental group; 100 non-GDM patients were selected as control group. High-performance liquid chromatography (HPLC) was used to determine vitamin A and E levels; high-performance liquid chromatography tandem mass spectrometer(HPLC-AS/MS) was used to determine 25-hydroxyvitamin D levels. Results: There was no significant difference in serum vitamin A levels between GDM patients and non-GDM patients (P>0.05); the serum 25-hydroxyvitamin D levels in GDM patients were significantly lower than those in the control group (P<0.05); the serum vitamin E levels in GDM patients were significantly higher than those in the control group (P<0.05). The serum level of 25-hydroxyvitamin D in patients with GDM was negatively correlated with the expression of vitamin E (r=-0.351, P<0.05). Conclusion: The level of 25-hydroxyvitamin D in patients with GDM is low, and the level of vitamin E is high, and there is a certain correlation between the two, and they may jointly participate in the development of GDM.