不同亚型脂联素与非酒精性脂肪性肝病的关系

目的:探究总脂联素(total adiponectin,total APN)和高分子量脂联素(high-molecular-weight adiponectin,HMW APN)与非酒精性脂肪性肝病(Nonalcoholic Fatty Liver Disease,NAFLD)的关系。方法:连续纳入50名男性健康男性及50名非酒精性脂肪性肝病男性患者,收集患者临床资料及其他临床生化数据,通过ELISA法检测总脂联素、支链氨基酸及可溶性晚期糖基化终末产物含量,Western blot法测定高分子量、中分子量和低分子量脂联素水平,进一步分析其相关性。结果:与对照组的健康受试者相比,NAFLD患者的总脂联素和三种不同形式的脂联素水平均显著降低。在NAFLD患者中,总脂联素与身高(R=-0.270, P=0.032)和羧甲基赖氨酸(R=-0.259, P=0.040)显著负相关;高分子量脂联素与空腹血糖(R=0.350, P=0.016)显著正相关,与丙氨酸氨基转移酶(R=-0.321, P=0.029)和天门冬氨酸氨基转移酶(R=-0.295, P=0.045)显著负相关。结论:总脂联素和三种不同形式的脂联素水平均与NAFLD呈显著负相关。

The Correlation between Different Adiponectin Isoforms with Nonalcoholic Fatty Liver Disease

ABSTRACT Objective: To investigate the correlation between total adiponectin (total APN) and high-molecular-weight adiponectin (HMW APN) with nonalcoholic fatty liver disease(NAFLD). Methods: A total of 50 healthy males and 50 male patients with NAFLD were included in the study. Their clinical biochemical characteristics were measured and collected. Total adiponectin, branched chain amino acid (BCAAs) and soluble receptor for advanced glycation end product (sRAGE) were measured by ELISA. The variation of high-molecular-weight, medium-molecular-weight and low-molecular-weight adiponectin levels were determined by Western blot in NAFLD patients. Then, the correlation among the characteristics was further analyzed. Results: When compared with healthy subjects in control group, significantly decreased levels of total adiponectin and three adiponectin isoforms were found in NAFLD patients. In NAFLD patients, height (R=-0.270, P=0.032) and N-epsilon carboxymethyl lysine (CML) (R=-0.259, P=0.040) were significantly negatively correlated with total adiponectin; HMW adiponectin was significantly positively associated with fasting plasma glucose (FPG) (R=0.350, P=0.016), and negatively associated with ALT (R=-0.321, P=0.029) and AST (R=-0.295, P=0.045) respectively. Conclusion: The levels of total adiponectin and three adiponectin isoforms are in significantly negative correlation with NAFLD.