两种农药对青鳉抗氧化酶活性和bcl6b基因表达的影响

为了解阿特拉津和毒死蜱对青鳉(Oryzias latipes)肌肉组织中抗氧化酶活性和B细胞淋巴瘤6B基因(B cell CLL/lymphoma 6B,bcl6b)表达的影响,实验通过不同染毒浓度的阿特拉津、毒死蜱,二者单独或共同染毒,在不同的染毒时间检测其对青鳉肌肉组织中超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-PX)活性及bcl6b mRNA表达的影响。结果表明:青鳉肌肉组织中的抗氧化酶(SOD,GSH-PX)活性随染毒农药浓度的升高而显著下降,bcl6b mRNA相对表达则显著升高;30 d恢复处理后,与染毒末期相比,恢复末期中浓度组和高浓度组的这两种酶活性显著升高,bcl6b mRNA表达则显著降低。这一结果证实该两种农药单独或共同染毒,会对青鳉产生毒性作用,使青鳉抗氧化酶(SOD,GSHPX)活性和bcl6b mRNA表达发生显著变化,bcl6b mRNA表达与抗氧化酶水平的变化趋势相反;环境条件改善后,上述2种酶的活性和bcl6b mRNA表达会得到有效恢复。

Effects of two kinds of pesticides on activities of antioxidant enzymes and the expression of bcl6b gene in Oryzias latipes

To clarify the effects of atrazine and chlorpyrifos on the activities of antioxidant enzyme and the gene expression of B cell CLL/lymphoma 6 B(bcl6 b)in muscle of Oryzias latipes,we measured superoxide dismutase(SOD)activity,glutathione peroxidase(GSH-PX)activity,and bcl6 b mRNA expression with Oryzias latipes being treated by exposure to different concentrations of atrazine and chlorpyrifos alone or together at different periods.The results showed that activities of antioxidant enzyme(SOD,GSH-PX)in muscle of Oryzias latipes significantly decreased with increasing pesticide concentration,while the relative expression of bcl6 b mRNA significantly increased.After recovered for 30 days,the activities of both antioxidant enzymes(SOD,GSH-PX)were significantly increased as compared to their counterparts at the end of recovery in the moderate concentration or high concentration group,while the expression of bcl6 b mRNA was significantly decreased after recovery.These results suggest that the exposure of Oryzias latipes to single or combined atrazine and chlorpyrifos had toxic effect,causing significant changes in the activities of antioxidant enzyme(SOD,GSH-PX)and the expression of bcl6 b mRNA.The expression of bcl6 b mRNA was contrary to the change trend of antioxidant enzyme levels.After the environmental conditions being improved,the activities of those two enzymes and the expression of bcl6 b mRNA could be effectively recovered.