脑出血大鼠血肿周围脑组织含水量与MMP-9、IL-6及TIMP-1表达水平的相关性研究

目的:研究脑出血(ICH)大鼠血肿周围脑组织含水量与基质金属蛋白酶-9(MMP-9)、白细胞介素-6(IL-6)及组织基质金属蛋白酶抑制剂-1(TIMP-1)表达水平的相关性。方法:84只健康成年雄性Wistar大鼠按随机数字表法分成观察组、假手术组以及对照组,每组28只。另将观察组分成ICH后1d亚组9只,3d亚组9只,7d亚组10只。观察组大鼠实施ICH模型的制备,假手术组的大鼠仅给予空针穿刺,对照组不进行模型制备。检测并对比各组血肿周围的脑组织含水量与MMP-9、IL-6、TIMP-1水平,对比观察组内不同亚组(ICH后1d、3d、7d)血肿周围的脑组织含水量、MMP-9、IL-6和TIMP-1水平,并分析ICH大鼠血肿周围的脑组织含水量与MMP-9、IL-6、TIMP-1表达水平的相关性。结果:观察组血肿周围的脑组织含水量与MMP-9、IL-6、TIMP-1水平均分别高于假手术组及对照组,差异均有统计学意义(P0.05)。观察组内3d和7d亚组血肿周围的脑组织含水量与MMP-9、IL-6、TIMP-1水平均分别高于1d亚组,但7d亚组低于3d亚组,差异均有统计学意义(P0.05)。根据Spearman相关性分析结果显示,ICH大鼠血肿周围的脑组织含水量与MMP-9、IL-6、TIMP-1表达水平均呈正相关(P0.05)。结论:ICH大鼠的血肿周围脑组织含水量与MMP-9、IL-6及TIMP-1表达水平均呈正相关,MMP-9、IL-6、TIMP-1在ICH后周围组织水肿的发生、发展中具有重要作用。

Correlations between the Water Content of Brain Tissue Around Hematoma and the Expression Levels of MMP-9, IL-6 and TIMP-1 in Rats with Intracerebral Hemorrhage

ABSTRACT Objective: To investigate the correlations between the water content of brain tissue around hematoma and the expression levels of matrix metalloproteinases-9 (MMP-9), interleukin-6 (IL-6) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in rats with intracerebral hemorrhage (ICH). Methods: 84 healthy adult male Wistar rats were randomly divided into observation group, sham-operated group and control group, with 28 rats in each group. In addition, the observation group was divided into 9 subgroups on the 1d after ICH, 9 subgroups on the 3d and 10 subgroups on the 7d. The ICH model was prepared in observation group, sham-operated group was only given empty needle puncture, while no model preparation for control group.The brain water content and MMP-9, IL-6 and TIMP-1 levels around hematoma in each group were detected and compared. The brain water content, MMP-9, IL-6 and TIMP-1 levels around hematoma in different subgroups of the observation group (1, 3 and 7 days after ICH) were compared. The relationships between brain water content around hematoma and the expression levels of MMP-9, IL-6 and TIMP-1 in ICH rats were analyzed. Results: The water content of brain tissue around hematoma and the levels of MMP-9, IL-6 and TIMP-1 in the observation group were higher than sham operation group and the control group, the differences were statistically significant (P<0.05). The water content of brain tissue around hematoma and the levels of MMP-9, IL-6 and TIMP-1 in the 3d and 7d subgroups in the observation group were higher than those in the 1d subgroup, but the 7d subgroup were lower than that in the 3d subgroup, the differences were statistically significant (P<0.05). According to Spearman correlation analysis, the brain water content around the hematoma of ICH rats was positively correlated with the expression levels of MMP-9, IL-6 and TIMP-1 (P<0.05). Conclusion: The water content of brain tissue around ICH rats are positively correlated with the expression levels of MMP-9, IL-6 and TIMP-1. MMP-9, IL-6 and TIMP-1 play an important role in the occurrence and development of peripheral tissue edema after ICH.