清肺化痰逐瘀汤治疗慢性阻塞性肺疾病模型大鼠的疗效及机制研究

目的:探讨清肺化痰逐瘀汤治疗慢性阻塞性肺疾病(COPD)大鼠的疗效及作用机制。方法:将60只SD大鼠按照随机数字表法分为空白对照组、模型对照组、低剂量组、中剂量组及高剂量组,每组各12只。空白对照组大鼠每天以生理盐水灌胃,将其他四组大鼠建立COPD模型,模型建立后,模型对照组每天以生理盐水灌胃,低剂量组、中剂量组、高剂量组分别以2 m L/100 g·d、3 m L/100 g·d、4 m L/100 g·d的清肺化痰逐瘀汤灌胃,各组均持续14d。比较各组大鼠症状改善情况、动脉血中氧分压(PO2)、二氧化碳分压(PCO2)以及血清白介素-1β(IL-1β)、白介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)、白三烯B4(LTB4)及超氧化物歧化酶(SOD)水平。结果:低剂量组、中剂量组、高剂量组大鼠饮食、精神、毛发等状况均有所改善,且高剂量组大鼠症状缓解最为明显。模型对照组、低剂量组、中剂量组、高剂量组大鼠血清IL-1β、IL-8、TNF-α、LTB4及PCO2水平均明显高于空白对照组,血清SOD及PO2水平明显低于空白对照组(P<0.05);且模型对照组、低剂量组、中剂量组、高剂量组大鼠血清IL-1β、IL-8、TNF-α、LTB4及PCO2水平逐渐降低,血清SOD及PO2水平逐渐升高(P<0.05)。结论:清肺化痰逐瘀汤能明显改善COPD大鼠症状和动脉血气,疗效确切,且高剂量清肺化痰逐瘀汤改善作用最明显,其可能机制是通过降低大鼠炎症因子及LTB4水平,提高SOD水平,从而抑制其机体炎症反应,增强抗氧化能力。

Effect and Mechanism of Qingfei Huatan Zhuyu Decoction on Chronic Obstructive Pulmonary Disease in Rats

Objective:To explore effect and mechanism of Qingfei Huatan Zhuyu Decoction on chronic obstructive pulmonary disease(COPD)in rats.Methods:A total of 60 SD rats were randomly divided into the blank control group,the model control group,the low dose group,the middle dose group and the high dose group respectively,12 in each group.Rats in the blank control group were intragastrically administered with saline every day.After the establishment of COPD model in the other four groups,rats in the model control group were intragastrically administered with normal saline every day,while rats in the low dose group,the middle dose group and the high dose group were intragastrically administered with Qingfei Huatan Zhuyu Decoction of 2 m L/100 g·d,3 m L/100 g·d and4 m L/100 g·d,respectively,each group lasted for 14 days.The improvement of symptoms,partial pressure of oxygen(PO2),partial pressure of carbon dioxide(PCO2)in arterial blood and serum levels of interleukin-1β(IL-1β),interleukin-8(IL-8),tumor necrosis factor-α(TNF-α),leukotriene B4(LTB4)and superoxide dismutase(SOD)were compared.Results:The diet,spirit and hair of rats in the low dose group,the middle dose group and the high dose group were improved,and the symptoms of rats in the high dose group were relieved most obviously.The serum levels of IL-1β,IL-8,TNF-α,LTB4 and PCO2 in model control group,low dose group,middle dose group,high dose group were significantly higher than those in blank control group,and the serum levels of SOD and PO2 were significantly lower than those in blank control group(P<0.05).The serum levels of IL-1β,IL-8,TNF-α,LTB4 and PCO2 in model control group,low-dose group,middle-dose group and high-dose group were decreased gradually,while the serum levels of SOD and PO2 were increased gradually(P<0.05).Conclusion:Qingfei Huatan Zhuyu Decoction can significantly improve the symptoms and arterial blood gas of COPD rats,with definite curative effect,High dose Qingfei Huatan Zhuyu Decoction has the most obvious improvement effect.Its possible mechanism is to reduce the levels of inflammatory factors and LTB4 in rats and increase the level of SOD,thereby inhibiting the body’s inflammatory response and enhancing the antioxidant capacity.