血清LncRNA XIST与miR-33a在膀胱癌中的表达及临床意义

目的:探究血清长链非编码RNA X染色体失活特异转录本(Lnc RNA XIST)与微小RNA-33a(mi R-33a)在膀胱癌中的表达及临床意义。方法:选择2017年4月至2019年8月青岛大学附属医院诊治的95例膀胱癌患者作为膀胱癌组,选择同期体检的95例健康者作为健康组。采用荧光定量PCR检测两组的血清LncRNA XIST与mi R-33a表达水平,分析患者的临床病理参数与血清LncRNA XIST、mi R-33a表达水平的关系,采用受试者工作特征曲线(ROC)分析血清LncRNA XIST与mi R-33a对膀胱癌的预测价值。结果:与健康组相比,膀胱癌组的血清LncRNA XIST表达水平明显升高(P0.05),而血清mi R-33a表达水平明显下降(P0.05)。血清LncRNA XIST与mi R-33a表达均与膀胱癌患者的TNM分期和淋巴结转移情况相关(P0.05)。血清LncRNA XIST联合mi R-33a(AUC=0.830,敏感性=90.47%,特异性=89.85%)预测膀胱癌的临床价值明显高于血清LncRNA XIST(AUC=0.716,敏感性=81.36%,特异性=80.74%)及血清mi R-33a(AUC=0.736,敏感性=82.19%,特异性=81.09%)单独检测。结论:血清LncRNA XIST表达异常升高、血清mi R-33a表达降低与膀胱癌发生密切相关,联合检测有助于预测膀胱癌发生的风险,从而为临床制定针对性干预和治疗方案提供参考。

Expression of Serum LncRNA XIST and miR-33a in Bladder Cancer and Its Clinical Significance

ABSTRACT Objective: To investigate the expression and clinical significance of serum Long Non-coding RNA XIST(LncRNA XIST) and microRNA-33a(miR-33a) in bladder cancer. Methods: 95 cases of patients with bladder cancer diagnosed and treated in Affiliated Hospital of Qingdao University from April 2017 to August 2019 were selected as the bladder cancer group, 95 healthy cases during the same period were selected as the healthy group. Serum LncRNA XIST and miR-33a express levels in the two groups were detected by fluorescence quantitative PCR, the relationship between the clinical pathological characteristics of the patients and serum LncRNA XIST and miR-33a express levels was analyzed, the diagnostic value of serum LncRNA XIST and miR-33a in bladder cancer was analyzed by ROC(receiver operating characteristics) curve. Results: Compared with the healthy group, the serum LncRNA XIST express level in the bladder cancer group was significantly increased (P<0.05), while the serum miR-33a level was significantly decreased (P<0.05). Both serum LncRNA XIST and miR-33a expression were associated with TNM staging and situation of lymph node metastasis in patients with bladder cancer (P<0.05). The clinical value of serum LncRNA XIST combined with miR-33a (AUC=0.830, sensitivity=90.47%, specificity=89.85%) in predicting bladder cancer was significantly higher than that of serum LncRNA XIST(AUC=0.716, sensitivity=81.36%, specificity=80.74%) and serum miR-33a(AUC=0.736, sensitivity=82.19%, specificity =81.09%) individual. Conclusion: Abnormal elevation of serum LncRNA XIST expression, decreased expression of serum miR-33a are closely related to the occurrence of bladder cancer, combined detection is helpful to predict the risk of bladder cancer, so as to provide reference for the development of targeted intervention and treatment programs.