蚓激酶对人瘢痕疙瘩成纤维细胞的生物学功能和MMP2、MMP9表达的影响

目的:考察蚓激酶对人瘢痕疙瘩成纤维细胞(KF)生物学功能影响,初步阐明其防治瘢痕疙瘩作用机制。方法:选取人KF为体外实验模型,随机分为对照组和蚓激酶低、中、高剂量组;应用药物干预48 h后,采用CCK8法测定人KF增殖率,流式细胞术检测凋亡率,划痕法测迁移能力,Western blot检测MMP2、MMP9蛋白表达。结果:与对照组相比,应用5 U·mL~(-1)、10 U·mL~(-1)和20U·mL~(-1)蚓激酶溶液干预后,人KF增殖率明显降低,凋亡率显著升高,在一定范围内与剂量成正相关性;与对照组相比,蚓激酶组的划痕距离均有不同程度地增加,MMP2、MMP9表达也显著下降,均有显著性差异。结论:蚓激酶可调节人KF的生物学功能,通过抑制细胞增殖和迁移、诱导凋亡、下调迁移相关蛋白MMP2、MMP9表达,防止瘢痕疙瘩的生长和侵润,为蚓激酶的进一步开发和利用提供有力支持。

The Biological Function of Lumbrokinase on Keloid Fibroblast and Its Expression MMP2, MMP9

ABSTRACT Objective: To investigate the effect of lumbrokinase(LBK) on the biological function of keloid fibroblast(KF), and to clarify mechanisms of prevention and treatment on Keloid. Methods: KF was selected as the experimental model and randomly divided into the control group and LBK low, medium, and high dose group; After 48 hours of treatment with drug, the proliferation rate of KF was measured by CCK8 method, apoptosis rate by flow cytometry, migration ability by scratch method, expression of MMP2 and MMP9 by western blot. Results: Compared with the control group, after the treatment with LBK, the proliferation rate of human KF decreased significantly, apoptosis rate and the scratch distance increased to varying degrees, there is a positive correlation with the dose to a certain extent. Compared with the control group, the expression of MMP2 and MMP9 decreased significantly, which was statistically significant. Conclusion: LBK can regulate the biological function of Human KF by inhibiting cell proliferation and Migration, inducing apoptosis, down-regulating the expression of MMP2 and MMP9, and preventing the growth and invasion of keloid, it provides strong support for the further development and utilization of LBK.