Application of electrospray ionization mass spectrometry in rapid typing of fengycin homologues produced by Bacillus subtilis |
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Authors: | Wang J Liu J Wang X Yao J Yu Z |
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Institution: | Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Science, Hefei, Anhui, China. wangjun0457@sina.com |
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Abstract: | AIMS: To rapidly type the fengycin homologues produced by Bacillus subtilis strains with electrospray ionization/collision-induced dissociation (ESI/CID) mass spectrometry. METHODS AND RESULTS: Fengycin homologues produced by Bacillus subtilis JA were analysed. When each homologue was subjected to ESI/CID analysis, ions representing characteristic fragmentations were detected. These ions can help to identify the homologues; even homologues of the same nominal mass can be discriminated by their ESI/CID spectra. Based on the CID results, fengycin homologues can be correctly assigned. CONCLUSIONS AND SIGNIFICANCE OF THIS STUDY: ESI/CID leads to rapid detection and structural characterization of fengycin homologues or lipopeptides with similar properties. It will be very useful in studying the regulatory expression of these peptides. |
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Keywords: | Bacillus subtilis electrospray ionization mass spectrometry fengycin typing |
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