Structure of Shigella sonnei lipid A |
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Authors: | URamadas Bath Tivadar Kontrohr Hubert Mayer |
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Institution: | Max-Planck-Institut für Immunbiologie, Freiburg, F.R.G.;Institute of Microbiology, University of Pécs, Pécs, Hungary |
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Abstract: | The detailed chemical structure of lipid A of Shigella sonnei phase II was elucidated. The lipid A backbone consists of a β-1,6-linked glucosamine disaccharide substituted with (mono) phosphates both at C-1 and C-4′. This was shown by selective degradation followed by 31P-NMR studies. C-4 and C-6′ were found to contain unsubstituted hydroxyl groups, the latter being the point of attachment of KDO as reported for other enterobacterial lipids A.The amino groups of the glucosamine disaccharide are substituted by 3-hydroxy fatty acids: 3-O-(14:0) 14:0 at the non-reducing glucosamine and 3-O-(12:0) 14:0 at the reducing glucosamine. In contrast to earlier reports, no ethanolamine or phosphodiester linkages were found in lipid A. |
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Keywords: | Shigella sonnei Lipid A (Structure) |
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