Glycine uptake by trout (Salmo trutta) red blood cells |
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Authors: | M. A. Gallardo J. Sänchez |
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Affiliation: | (1) Dept. Bioquímica i Fisiologia, Unitat de Fisiologia, Universitat de Barcelona, E-08028 Barcelona, Spain |
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Abstract: | The present study demonstrates the presence of different amino acid carriers in the membrane of trout red cells. Most glycine is taken up through the Na+-dependent system ASC, although the nearly specific Gly system is also active. Besides these carriers, glycine is taken up by means of Na+-independent transporters, system l being the most important. A system asc of high affinity and low capacity has been found, and band 3 is unable to transport glycine under physiological conditions. These results suggest that although all these carriers are already present in primitive vertebrates, several differences exist in their properties with respect to those found in mammalian cells.We would like to express our sincere thanks to Mr. Antonino Clemente (Piscifactoria de Bagà, Medi Natural, Generalitat de Catalunya) for his help and logistical assistance and to Mr. Robin Rycroft for his editorial help.This work was supported by a grant of Comisió Interdepartamental de Recerca i Technologia (AR90-3.3394). M.A.G. is recipient of a fellowship from the Generalitat de Catalunya. |
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Keywords: | Glycine uptake Trout (Salmo truttd) Red blood cells |
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