Identification and Phytotoxicity of 3-methylthiopropanoic and Trans-3-methylthiopropenoic Acids Produced in Culture by Xanthomonas campestris pv. vitians |
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Authors: | F Scala A Zoina L Coppola R Capasso M Lorito A Evidente |
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Institution: | Istituto di Patologia Vegetale, Universita di Napoli, Federico II, 80055 Portici, Italy;Dipartimetito di Scienze Chimico-Agrarie, Universita di Napoli, Federico II, 80055 Portici, Italy |
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Abstract: | Two phytotoxic metabolites were isolated from culture filtrates of Xanthomonas campestris pv, vitians , the causal agent of lettuce leaf spots and headrot. The two compounds were identified as 3-methylthiopropanoic (1) and trans-3-methylthiopropenoic (2) acids by chemical and spectroscopic methods. Toxic effects of the two compounds on leaf tissues and protoplasts of lettuce and cabbage were investigated. Solutions of 1 and 2 induced chlorosis and necrosis on lettuce leaves at minimum concentrations of 300 and 50 μg/ml, respectively. Infiltration in cabbage leaves did not produce any symptoms. The LD50 values for 1 and 2 against lettuce protoplasts were 15 and 16 μg/ml, respectively. Activity of the two metabolites against cabbage protoplasts was very low (LD50 > 500 μg/ml). |
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