烟草与枸杞叶片组织培养中的无丝分裂

AMITOSIS IN THE LEAVES OF NICOTIANA TABACUM L. AND LYCIUM BARBARUM L. DURING TISSUE CULTURE

The present paper reports amitosis phenomenon in the leaves of Nicotiana tabacurn L. and Lycium barbarum L. during tissue culture. The mode of amitosis has three kinds, contriction, cleavege and fragmentation. The first amitosis occurred before mitosis came, with the continuation of cultured time, amitosis number increased gradually, but amitosis decreased gradually and came to disappearance when the high tide of mitosis arrived. Amitosis always took place in the cells of mesophyll near the bundle. This is also one part of calluse formation and buds differentiation. Therefore, it is inferred that the amitosis in explants during the early stage of tissue cultures may lead to chromosome variation in cultured cells, thus influencing genetics stability of regeneration plantlets.