The cytokinins of cultured carrot cells |
| |
| Authors: | S Salem D Linstedt J Reinert |
| |
| Institution: | (1) Institut für Pflanzenphysiologie und Zellbiologie, Fachbereich Biologie, Freie UniversitÄt Berlin, Königin-Luise-Strasse 12-16 a, D-1000 Berlin 33;(2) Present address: Faculty of Science, Botany Department, Ain-Shams University, Cairo, Egypt |
| |
| Abstract: | Summary Cytokinin-autotrophic strains of carrot callus contained active substances with Chromatographic mobilities on Sephadex LH-20 corresponding to 6-(4-hydroxy-3-methylbut-trans-2-enylamino)purine (Zeatin, Z), 6-(3-methylbut-2-enylamino)purine (iP) and the ribosides (9R)Z and (9R)iP. The apparent major activity was found in a fraction, with an elution volume of 242–291 ml. Hydrolysis of this fraction with HCl and  -Glucosidase gave rise to Z, indicating that the major active compound is a polar conjugate of Zeatin.In all experiments the extracts were tested immediately after preparation; deep freeze storage, led to a considerable loss of activity in polar fractions. while the free base cytokinins and their ribosides showed increased activity levels.Analogous results were obtained by means of paper chromatography. |
| |
| Keywords: | Cytokinins Daucus carota L Plant tissue culture |
| 本文献已被 SpringerLink 等数据库收录！ |
|
