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High‐resolution elemental localization in vacuolate plant cells by nanoscale secondary ion mass spectrometry |
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| Authors: | Katharine E. Smart J. Andrew C. Smith Matt R. Kilburn Barrie G. H. Martin Chris Hawes Chris R. M. Grovenor |
| Affiliation: | 1. Department of Materials, University of Oxford, Parks Road, Oxford, OX1 3PH, UK;2. Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK;3. Present address: Centre for Microscopy, Characterisation and Analysis M010, The University of Western Australia, Crawley, WA 6009, Australia.;4. School of Life Sciences, Oxford Brookes University, Gipsy Lane, Oxford, OX3 0BP, UK |
| Abstract: | By combining the capabilities of advanced sample preparation methodologies with the latest generation of secondary ion mass spectrometry instrumentation, we show that chemical information on the distribution of even dilute species in biological samples can be obtained with spatial resolutions of better than 100 nm. Here, we show the distribution of nickel and other elements in leaf tissue of the nickel hyperaccumulator plant Alyssum lesbiacum prepared by high‐pressure freezing and freeze substitution. |
| Keywords: | NanoSIMS elemental localization high‐pressure freezing hyperaccumulator nickel vacuole |