Characterization of Fusarium oxysporum f.sp. cepae from Onion in Turkey Based on Vegetative Compatibility and rDNA RFLP Analysis |
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| Authors: | Harun Bayraktar Muharrem Türkkan Fatma Sara Dolar |
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| Institution: | 1. Authors’ addresses: Department of Plant Protection, Faculty of Agriculture, University of Ankara, 06110, Ankara, Turkey;2. Department of Plant Protection, Faculty of Agriculture, University of Ordu, 52200, Ordu, Turkey (correspondence to H. Bayraktar. E‐mail: bayrakta@agri.ankara.edu.tr) |
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| Abstract: | Seventy‐five isolates of Fusarium oxysporum f.sp. cepae, the causal agent of basal plate rot on onion, were obtained from seven provinces of Turkey. The isolates were characterized by vegetative compatibility grouping (VCGs) and restriction fragment length polymorphism (RFLP) analysis of the nuclear ribosomal DNA intergenic spacer region (IGS). Forty‐eight vegetative compatibility groups were found, each containing a single isolate. Only one isolate formed strong heterokaryons with the reference isolates of VCG 0423. Five isolates were heterokaryon self‐incompatible. Restriction fragment analysis with six different enzymes revealed 13 IGS types among 75 F. oxysporum isolates from Turkey as well as 16 reference isolates from Colorado, USA. The majority of single‐member VCGs produced identical RFLP banding patterns with minor deviations, considerably different from those of the reference VCG isolates. These results suggested that isolates of F. oxysporum f.sp. cepae in Turkey derived from distinct clonal lineages and mutations at one or more vegetative compatibility loci restrict heterokaryon formation. |
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| Keywords: | onion Allium cepa L Fusarium basal rot VCG PCR– RFLP |
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