NMR studies of a bacterial cell culture medium (LB Broth): cyclic nucleosides in yeast extracts |
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| Authors: | Michael H Rayner Peter J Sadler Michael D Scawen |
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| Institution: | Department of Chemistry, Birkbeck College, Gordon House, Laboratory, London, U.K. |
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| Abstract: | The composition of LB broth (tryptone, yeast extract and NaCl) was investigated by 1H,31P-NMR spectroscopy, FPLC and gel electrophoresis. An unexpected finding was the high level of 2'3'-cyclic nucleotides, detected by characteristic 31P-NMR resonances in the region 20-21 ppm, originating from the yeast component. 31P-NMR resonances for cyclic nucleotides were observed during the autolysis of Saccharomyces cerevisiae cells, and in model reactions of RNase with RNA. |
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| Keywords: | 1H 31P-NMR LB broth Yeast autolysis Yeast extracts Cyclic nucleosides |
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