Cloning of chromosomal beta-lactamase genes from Yersinia enterocolitica |
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| Authors: | A Seoane J M García Lobo |
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| Institution: | Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain. |
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| Abstract: | Two beta-lactamase genes present in the chromosome of Yersinia enterocolitica have been cloned individually into the plasmid pACY184 and expressed in Escherichia coli. The gene for broad-spectrum beta-lactamase I ('A') was cloned from a strain belonging to the O:3 serotype, and the gene for (cephalosporinase) beta-lactamase II ('B') was cloned from a strain of the O:5b serotype. The properties of the beta-lactamases expressed in E. coli are similar to those previously described in Y. enterocolitica. |
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