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Toward closing rice telomere gaps: mapping and sequence characterization of rice subtelomere regions
Authors:Tae-Jin Yang  Yeisoo Yu  Song-Bin Chang  Hans de Jong  Chang-Sik Oh  Sang-Nag Ahn  Eric Fang  Rod A Wing
Institution:(1) Brassica Genomics Team, National Institute of Agricultural Biotechnology, RDA, Suwon, 441-707, Korea;(2) Arizona Genomics Institute, University of Arizona, 303 Forbes building, Tucson, AZ 85721, USA;(3) Laboratory of Genetics, Wageningen University, Wageningen, The Netherlands;(4) Department of Agronomy, ChungNam Natrsquol University, Daejeon, Korea;(5) Clemson University, Clemson, SC 29760, USA
Abstract:Despite the collective efforts of the international community to sequence the complete rice genome, telomeric regions of most chromosome arms remain uncharacterized. In this report we present sequence data from subtelomere regions obtained by analyzing telomeric clones from two 8.8 × genome equivalent 10-kb libraries derived from partial restriction digestion with HaeIII or Sau3AI (OSJNPb HaeIII and OSJNPc Sau3AI). Seven telomere clones were identified and contain 25–100 copies of the telomere repeat (CCCTAAA)n on one end and unique sequences on the opposite end. Polymorphic sequence-tagged site markers from five clones and one additional PCR product were genetically mapped on the ends of chromosome arms 2S, 5L, 10S, 10L, 7L, and 7S. We found distinct chromosome-specific telomere-associated tandem repeats (TATR) on chromosome 7 (TATR7) and on the short arm of chromosome 10 (TATR10s) that showed no significant homology to any International Rice Genome Sequencing Project (IRGSP) genomic sequence. The TATR7, a degenerate tandem repeat which is interrupted by transposable elements, appeared on both ends of chromosome 7. The TATR10s was found to contain an inverted array of three tandem repeats displaying an interesting secondary folding pattern that resembles a telomere loop (t-loop) and which may be involved in a protective function against chromosomal end degradation.Electronic Supplementary Material Supplementary material is available for this article at
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