Application of accelerated storage test to lyophilized plant viruses

Summary The preservation of nine plant virus strains of tobamovirus and cucumovirus groups after freeze-drying in different lyophilic forms was examined. Quantitative studies on survival were performed. In tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV), accelerated storage test at 70 – 100°C was applied for screening 20 protecting media. A perspective medium, 5% sorbitol, 3.6% dextran, for plant viruses lyophilization with high cryo- and xeroprotective effects was found.