Increase of embryogenic callus formation in cucumber by initial culture on high concentration of 2,4-dichlorophenoxyacetic acid |
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Authors: | Anne-Marie Kuijpers Han Bouman Geert-Jan de Klerk |
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Institution: | (1) Centre for Plant Tissue Culture Research, PO Box 85, 2160 AB Lisse, The Netherlands |
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Abstract: | Cucumber (Cucumis sativus L.) leaf explants were cultured either continuously on standard medium containing 4.5 µM 2,4- dichlorophenoxyacetic acid (2,4-d) and 4.4 µM benzylaminopurine, or first cultured for various periods at different levels of 2,4-d, picloram or naphthaleneacetic acid (NAA), and then transferred to standard medium. When cultured continuously on standard medium, less than 10% of the explants formed embryogenic callus. Initial culture on picloram or NAA, or on 2,4-d at a low concentration (1.4 µM) did not result in any embryogenic callus formation. Embryogenic callus formation increased to 40% if during the initial phase of the culture (10 days), the 2,4-d concentration was raised to 14 µM. Prolonged culture on 14 µM 2,4-d resulted in less embryogenic callus formation.Abbreviations BA
benzylaminopurine
- 2,4-d
2,4-dichlorophenoxyacetic acid
- NAA
naphthaleneacetic acid |
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Keywords: | Auxins Cucumis regeneration somatic embryogenesis |
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