2,5-Diketogluconate formation byChromobacterium |
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| Authors: | M Bernaerts J De Ley |
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| Institution: | (1) Central Laboratory, Ministry of Economic Affairs, Brussels, Belgium;(2) Laboratory for Microbiology and Microbial Genetics, Faculty of Sciences, State University, Gent, Belgium |
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| Abstract: | Chromobacterium lividum oxidizes gluconate to 2,5-diketogluconate via 2-ketogluconate. The conversion by either growing or resting cells proceeds best in an aerated medium at pH 5.9. The yield is nearly 50%. Diketogluconate is not further decomposed. The preparation of chromatographically pure diketogluconate using eitherChromobacterium orGluconobacter is described. Several properties of the diketo compound are described. |
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