Influence of fetal bovine serum on cytotoxic and genotoxic effects of lectins in MCF-7 cells |
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Authors: | Faheina-Martins Glaucia Veríssimo da Silveira Alethéia Lacerda Ramos Márcio Viana Marques-Santos Luís Fernando Araujo Demetrius Antonio Machado |
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Affiliation: | 1. Departamento de Biologia Molecular, Universidade Federal da Paraíba, Jo?o Pessoa, Paraíba, CEP 58051‐900, Brazil;2. Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, Jo?o Pessoa, Paraíba, CEP 58051‐970, Brazil;3. Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, CEP 60455‐760, Brazil |
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Abstract: | Canavalia ensiformis (ConA), Canavalia brasiliensis (Conbr), and Cratylia floribunda (CFL) lectins have exhibited glucose-mannose binding specificity. We investigated the effect of fetal bovine serum (FBS) concentrations (1, 5, 10, and 20%) on the cytotoxic effect of these lectins against breast tumor cell line MCF-7. Cell viability was examined using the MTT reduction assay. When cells were grown in a medium supplemented with a higher serum concentration (10 or 20%), all lectins were much less toxic. When we used 1% FBS, it was possible to achieve a concentration-dependent activity by all examined lectins, with an IC(50) of 3.5, 25, and 60 μg/mL for ConA, Conbr, and CFL, respectively. All lectins incubated with 1% FBS induced apoptosis and DNA damage in MCF-7 cells. We conclude that ConA, Conbr, and CFL lectins' cytotoxic and genotoxic effects were observed only at low concentrations of serum. |
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Keywords: | Apoptosis Cytotoxicity Genotoxicity Fetal Bovine Serum Lectins |
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