Dissemination of entomopathogenic fungi using Busseola fusca male as vector |
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Authors: | Nguya K. Maniania,Elizabeth Ouna,Peter Ahuya,Brigitte Fré rot,Anne-Emmanuelle Fé lix,Bruno Le Ru,Paul-André Calatayud |
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Affiliation: | aThe International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772-00100, Nairobi, Kenya;bInstitut de Recherche pour le Développement (IRD) c/o ICIPE, P.O. Box 30772-00100, Nairobi, Kenya;cInstitut National de Recherche Agronomique (INRA), UMR PISC 1272, Route de St. Cyr, 78026 Versailles Cedex, France;dCentre National de la Recherche Scientifique (CNRS), Laboratoire Evolution, Genomes et Spéciation (LEGS), Av. De la Terrasse, BP1, 91198 Gif-sur-Yvette Cedex, France;eUniversité Paris-Sud 11, 91405 Orsay Cedex, France |
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Abstract: | The stem borer, Busseola fusca (Fuller), is an important pest of maize Zea mays L. and sorghum Sorghum bicolor (L.) in eastern and southern Africa. To control this pest, biological control methods including the use of entomopathogenic fungi are being considered. The pathogenicity of one isolate of Metarhizium anisopliae (Metsch.) Sorok. and one isolate of Beauveria bassiana Bals. (Vuill.) were first tested on different developmental stages of B. fusca including eggs, neonate, 2nd and 3rd-instar larvae. Both fungal isolates were pathogenic to all the stages tested. However, differences in mortality were observed among larvae that hatched from treated egg masses. Experiments were conducted thereafter to test whether B. fusca males could serve as a vector for fungal conidia to contaminate B. fusca females and subsequently eggs and larvae. Results demonstrated that B. fusca males successfully transferred inoculum to females during copulation, which in turn transmitted it to the eggs they laid on maize plants, resulting in the decrease of leaf damages. |
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Keywords: | Stem borer Busseola fusca Entomopathogenic fungi Metarhizium anisopliae Beauveria bassiana Autodissemination |
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