Colchicine enhances the effect of growth factors on the initiation of DNA synthesis in sparse and confluent Swiss 3T3 cells |
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| Affiliation: | 1. Department of Endocrinology, Wuhan General Hospital of Guangzhou Command, Wuluo Road 627, Wuhan 430070, Hubei Province, China;2. Department of Hematology and Medical Oncology, School of Medicine, Emory University, Atlanta, GA 30322, USA;1. Department of Gerontology, Qilu Hospital, Shandong University, Jinan 250012, China;2. Department of Life Science, Shandong Academy of Medical Science, Jinan 250062, China;3. Department of Cardiology, Shandong General Police Hospital, Jinan 250000, China;4. Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, China;1. Turretfield Research Centre, South Australian Research and Development Institute, Rosedale, SA 5350, Australia;2. Inverbrackie, Strathalbyn, SA 5255, Australia;3. Centre for Animal Science, Queensland Alliance for Agriculture and Food Innovation, and School of Veterinary Science, The University of Queensland, St Lucia, Qld 4067, Australia |
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| Abstract: | We have studied the effect of colchicine on the initiation of DNA synthesis stimulated by serum or by growth factors such as EGF, FGF or PGF2α in sparse and confluent resting Swiss 3T3 cells. Colchicine in combination with FGF or with low concentrations of serum is more effective in stimulating DNA synthesis than with EGF or PGF2α. Only in the presence of insulin do these latter two growth factors give with colchicine a response comparable to that of FGF with colchicine. This phenomenon was observed both in sparse and in confluent resting cells. We conclude that the synergistic effect of colchicine with growth factors or with serum does not depend on the cellular saturation density at which Swiss 3T3 cells become quiescent. |
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