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Acetylcholine in embryos of Oryzias latipes,a teleost: Gas chromatographic-mass spectrometric assay
Affiliation:1. College of Computer Science and Software Engineering, Shenzhen University, China;2. Department of Computer Science and Engineering, Southern University of Science and Technology, China;3. Shenzhen Institute of Artificial Intelligence and Robotics for Society, Shenzhen, China;4. National Laboratory of Pattern Recognition, Institute of Automation, Chinese Academy of Sciences, China;5. Watrix technology limited co. ltd, China;1. Solar Cell Applications Research Lab, Department of Physics, Government College University Lahore, Lahore, 54000, Punjab, Pakistan;2. Faculty of Veterinary and Animal Sciences, Muhammad Nawaz Shareef University of Agriculture, Multan, 66000, Punjab, Pakistan;3. Faculty of Pharmacy, The University of Lahore, Lahore, 54000, Pakistan;4. Core Research Facilities, Research Institute, King Fahd University of Petroleum & Minerals, Dhahran, 31261, Saudi Arabia
Abstract:1. A gas chromatographic-mass spectrometric assay was used to acetycholine in extracts of embryos of the medaka Oryzias latipes, a teleost fish.2. Acetylcholine is present during cleavage and in blastulae and gastrulae, the mean amount per embryo ranging from 0.49 to 1.43 pmol.3. A substantial proportion of the total acetylcholine in the embryo and yolk is present in cells dissociated from the embryo.
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