A novel type A2 neurotoxin gene cluster in Clostridium botulinum strain Mascarpone |
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Authors: | Franciosa Giovanna Maugliani Antonella Floridi Francesca Aureli Paolo |
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Institution: | National Center for Food Quality and Risk Assessment, Laboratory of Food Microbial Hazards, Istituto Superiore di Sanità, Rome, Italy. |
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Abstract: | The partial nucleotide sequence ( approximately 10 kb) of the cluster of genes encoding the botulinum neurotoxin complex in Clostridium botulinum type A strain Mascarpone was determined. The analysis revealed six ORFs (orfs), which were organized as in the type A2 and type A3 botulinum neurotoxin gene clusters of strains Kyoto-F and NCTC 2916, respectively. While the orfs at the proximal and distal ends of the sequence (orfX2 and bont/A genes) shared a high level of similarity with the corresponding sequences of strain Kyoto-F, the segment encompassing the orfX1 and botR/A genes within the sequence exhibited a higher degree of homology to the related region in strain NCTC 2916. The mosaic structure of the Mascarpone neurotoxin gene cluster suggests recombinational exchanges. |
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Keywords: | Clostridium botulinum boNT/A gene cluster nucleotide sequence |
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