Isolation and identification of N2-fixing microorganisms from the rhizosphere of Capparis spinosa (L.) |
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| Authors: | Andrade Galdino Esteban Eduardo Velasco Leonardo Lorite María J. Bedmar Eulogio J. |
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| Affiliation: | (1) Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC P.O. Box 419, 18080- Granada, Spain;(2) Present address: United States Department of Agriculture, Horticultural Crops Research Laboratory, 3420 NW Orchard Ave., Corvallis, OR 97333, USA;(3) Departamento de Agroecología y Protección Vegetal, Estación Experimental del Zaidín, CSIC P.O. Box 419, 18080- Granada, Spain |
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| Abstract: | Four bacterial strains, Pseudomonas stutzeri var. mendocina, Comamonas sp., Agrobacterium tumefaciens biovar. 2 and Sphingobacterium sp., isolated from the rhizosphere of wild-grown caper (Capparis spinosa L.) plants were able to fix N2 as shown by their growth in nitrogen-free medium and by the acetylene reduction test. P. stutzeri var. mendocina and Comamonas sp. contained DNA homologous to the Klebsiella pneumoniae M5a1 nifHDK genes. No hybridization was found with total DNA from either A. tumefaciens biovar. 2 or Sphingobacterium sp. using nifHDK probes from either K. pneumoniae or Rhizobium meliloti. |
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| Keywords: | Agrobacterium Capparis spinosa Comamonas N2 fixation Pseudomonas rhizosphere Sphingobacterium |
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