Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing |
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Authors: | Girish Aparna Sun Hongyan Yeo Dawn S Y Chen Grace Y J Chua Teck-Khiang Yao Shao Q |
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Institution: | Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore. |
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Abstract: | One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. Herein, we report a bacterium-based, intein-mediated strategy to generate N-terminal cysteine-containing proteins which are then chemoselectively immobilized to a thioester-functionalized glass slide to generate the corresponding protein microarray. We also showed preliminary data of the strategy in a yeast host system. |
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