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Characterisation of a |
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| Authors: | June Southall Sarah L. Henry Alastair T. Gardiner Aleksander W. Roszak William Mullen Anne-Marie Carey Sharon M. Kelly Claire Ortmann de Percin Northumberland Richard J. Cogdell |
| Affiliation: | 1.Institute of Molecular Cell and Systems Biology,University of Glasgow,Glasgow,Scotland, UK;2.Biomedical Engineering, School of Engineering,University of Glasgow,Glasgow,Scotland, UK;3.Institute of Cardiovascular and Medical Sciences,University of Glasgow,Glasgow,Scotland, UK;4.Center for Innovations in Medicine, The Biodesign Institute,Arizona State University,Tempe,USA |
| Abstract: | Rhodopseudomonas palustris is a species of purple photosynthetic bacteria that has a multigene family of puc genes that encode the alpha and beta apoproteins, which form the LH2 complexes. A genetic dissection strategy has been adopted in order to try and understand which spectroscopic form of LH2 these different genes produce. This paper presents a characterisation of one of the deletion mutants generated in this program, the pucBAd only mutant. This mutant produces an unusual spectroscopic form of LH2 that only has a single large NIR absorption band at 800 nm. Spectroscopic and pigment analyses on this complex suggest that it has basically a similar overall structure as that of the wild-type HL LH2 complex. The mutant has the unique phenotype where the mutant LH2 complex is only produced when cells are grown at LL. At HL the mutant only produces the LH1-RC core complex. |
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