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| 心肌特异性新蛋白激酶p93和peroxiredoxin 3的相互作用 | |
| 引用本文： | 冯艳,刘冬青,王震,曹慧青,时娜,邓仲端,丁金凤,孟宪敏.心肌特异性新蛋白激酶p93和peroxiredoxin 3的相互作用[J].生物化学与生物物理进展,2004,31(8):688-692. |
| 作者姓名： | 冯艳 刘冬青 王震 曹慧青 时娜 邓仲端 丁金凤 孟宪敏 |
| 作者单位： | 1. 上海同济医科大学病理教研室 2. 中国医学科学院,阜外心血管病医院分子医学中心,北京,100037;中国协和医科大学,阜外心血管病医院分子医学中心,北京,100037 3. 华中科技大学同济医学院药理系,武汉,430030 4. 华中科技大学同济医学院病理系,武汉,430030 |
| 基金项目： | 国家自然科学基金资助项目(30270557, 30300131)和北京市重大科技资助项目(H020220020310). |
| 摘 要： | 为了明确p93在心血管系统中的生物学功能及在信号传导通路中的作用，采用酵母双杂交技术，选择p93 N端第101～372位氨基酸构建诱饵质粒，筛选成人心脏cDNA文库，寻找与之相互作用的蛋白质，结果得到蛋白peroxiredoxin 3（PRX 3）.采用体外结合实验和免疫共沉淀等方法，证实了原核和真核表达的p93确实可与PRX3相互作用，通过双重荧光共定位，为二者的相互作用提供了定位依据.研究提示p93可能参与心肌细胞中与PRX3有关的各种生理病理过程，如生长、分化、发育、凋亡、氧应激等. |
| 关 键 词： | p93，peroxiredoxin 3，酵母双杂交，相互作用 |
| 收稿时间： | 2004/3/30 0:00:00 |
| 修稿时间： | 2004/4/28 0:00:00 |
Interaction Between The Novel Cardiac-specific Protein Kinase p93 and Peroxiredoxin 3 |
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| FENG Yan,LIU Dong-Qing,WANG Zhen,CAO Hui-Qing,SHI N,DENG Zhong-Duan,DING Jin-Feng and MENG Xian-Min.Interaction Between The Novel Cardiac-specific Protein Kinase p93 and Peroxiredoxin 3[J].Progress In Biochemistry and Biophysics,2004,31(8):688-692. | |
| Authors: | FENG Yan LIU Dong-Qing WANG Zhen CAO Hui-Qing SHI N DENG Zhong-Duan DING Jin-Feng and MENG Xian-Min |
| Institution: | Molecular Medical Center, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China;Department of Pathology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Molecular Medical Center, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China;Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Molecular Medical Center, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China;Molecular Medical Center, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China;Department of Pathology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Molecular Medical Center, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China;Molecular Medical Center, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China |
| Abstract: | |
| Keywords: | p93 peroxiredoxin 3 yeast two-hybrid interaction |
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