Reactivity of Limulus amoebocyte lysate towards (1----3)-beta-D-glucans |
| |
| Authors: | N Ohno Y Emori T Yadomae K Saito A Masuda S Oikawa |
| |
| Institution: | Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, Japan. |
| |
| Abstract: | The structure activity relationship for beta-D-glucans for the gelation of the amoebocyte lysates of the horseshoe crab (Limulus) has been investigated. beta-D-Glucans that had no (1----3) linkages induced little or no gelation. The (1----3)-beta-D-glucans curdlan (unbranched), grifolan (approximately 33% branched), schizophyllan (approximately 33% branched), lentinan (approximately 40% branched). SSG (approximately 50% branched), and OL-2 (approximately 66% branched) induced significant gelation. The optimum concentration for gelation was correlated with the content of branching. Single chain (rather than a triple helix) conformation and higher molecular weight were associated with higher reactivity. |
| |
| Keywords: | |
|
|
