Megacinogenic plasmid from Bacillus megaterium 216 |
| |
Authors: | K Rost?s S V Dobritsa A P Dobritsa C Koncz L Alf?ldi |
| |
Institution: | (1) Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, P. O. Box 521, H-6701 Szeged, Hungary;(2) Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of Sciences, 42292 Pushchino, Moscow Region, USSR |
| |
Abstract: | Summary Ability to produce megacin A, a bacteriocin of b. megaterium, was transferred from the strain B. megaterium 216 into auxotrophic derivatives of the strain B. megaterium KM via protoplast fusion and polyethylene-glycol-induced protoplast transformation by plasmid DNA, respectively. A 30.9 megadalton plasmid was detected in cells with MegA phenotype, and the loss of this phenotype was accompanied in each case with the elimination of that plasmid. The megacinogenic plasmid pBM309 has a single site for both BamIII and XhoI. It is cleaved by the endonucleases SalI, BglII, PstI, PvuII, and EcoRI into 3, 3, 4, 4, and 9 fragments, respectively. The physical map of this plasmid is presented. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|