| 辣椒疫霉产毒共分离RAPD标记的研究* |
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| 引用本文： | 谢丙炎 朱国仁. 辣椒疫霉产毒共分离RAPD标记的研究*[J]. 菌物学报, 2000, 19(1) |
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| 作者姓名： | 谢丙炎 朱国仁 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所 |
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| 摘 要： | 辣椒疫霉（Ｐｈｙｔｏｐｈｔｈｏｒａ ｃｏｐｓｉｃｉ）毒素的产生是由一个不完全显性基因所控制，通过ＲＡＰＤ／ＢＳＡ分析证明，用ＯＰＷ１２扩增得到一条约１３００ｂｐ的ＲＡＰＤ标记带，该带与辣椒疫霉产毒共分离。纯化回收ＯＰＷ１２ １３００ＤＮＡ，共转化感受态Ｅ．ｃｏｌｉ ＤＨ５ａ，筛选出３个白色阳性克隆，序列分析发现该标记ＤＮＡ为１２９１ｂｐ。这一标记ＤＮＡ序列的阐明为进一分析产毒遗传机理提供了新的信息。
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| 关 键 词： | 辣椒疫毒 毒素 共分离RAPD标记 |
COSEGREGATIVE RAPD MARKER OF TOXIN-PRODUCTIONOF PHYTOPHTHORA CAPSICI |
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| Abstract: | The results of bulk segregant analysis (BSA) / RAPD suggested that OPW121300 RAPD markerCosegregated with toxin production of Phytophthora capsici. This cosegregative RAPD marker was purified fromagaros gel electrophoresis of PCR amplication Products with OPW12, and cloned into PGEM-T-easy vector, threepositive clones identified by EcoR I degestion were further obtained from cloned vectors. DNA sequencing ofpositive clones shown that OPW121300 DNA length was 1291bp. |
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| Keywords: | Phytophthora capsici Toxin Cosegregative RAPD marker |
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