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Photooxidation by Photosystem II of Tris-washed Chloroplasts
Authors:Takashi Yamashita and  Warren L Butler
Institution:aDepartment of Biology, University of California, San Diego, La Jolla, California 92037
Abstract:Irradiation of tris-washed chloroplasts with moderate intensities of red light caused a partial bleaching of chloroplast pigments and an inhibition of the hydroquinone-supported photoreduction of NADP. The presence of an electron donor for photosystem 2 (PS2) during the irradiation prevented the bleaching and inhibition. It is concluded that the strong oxidant produced by PS2 accumulates in tris-washed chloroplasts during irradiation and an electron donor for PS2 protects against the photooxidation reactions.
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