Effects of nystatin on membrane conductance and internal ion activities inAplysia neurons |
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Authors: | John M Russell Douglas C Eaton Malcolm S Brodwick |
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Institution: | (1) Department of Physiology and Biophysics, University of Texas Medical Branch, 77550 Galveston, Texas |
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Abstract: | Summary Two methods were used to study effects of the antibiotic, nystatin, on giant neurons ofAplysia. In the first method the effects of various concentrations of nystatin on the current-voltage relationship were evaluated at a fixed time after exposure to the antibiotic using a two-microelectrode voltage clamp. Nystatin increased membrane conductance in a dose-dependent manner. The dose-response relation was very steep, with little or no effect below 15 mg/liter and an effect too large to measure at concentrations greater than 30 mg/liter. Upon return to antibiotic-free solution, membrane conductance returned to pre-treatment levels within 30 minutes. The second type of experiment involved use of ion-specific microelectrodes to measure changes of intracellular univalent ion activities which attended the nystatin-induced permeability. meability was also increased. Nystatin may therefore be used to selectively rearrange the internal ionic milieu to study the effect of such a change on membrane tranpsort or electrical properties. |
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