Studies on the production of indole-3-acetic acid with auxin-heterotrophic mutants derived from cultured crown gall cells |
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Authors: | Atsumi Shigeaki |
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Institution: | Department of Biology, College of General Education, University of Tokyo Komaba, Meguro-ku, Tokyo 153, Japan |
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Abstract: | Mutants in the indole-3-acetic acid metabolism derived fromcultured crown gall cells were tested to see whether they couldutilize any one of eight indolic compounds in place of indole-3-aceticacid. Two auxin-heterotrophic mutant cell lines could not utilizeindolepyruvic acid, but growth recovered when there was a supplementof indole-3-acetic acid. Indoleacetonitril and indoleacetaldoximeinhibited the growth of mutant cell lines and their parentalcrown gall cells. Cultured crown gall cells may have synthesizedindole-3-acetic acid from tryptophan via indolepyruvic acidand indole-acetaldehyde, and also may be able to produce indole-3-aceticacid from tryptophan via tryptamine (Received May 6, 1980; ) |
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