Molar mass distribution of hydroxypropyl cellulose by size exclusion chromatography with dual light scattering and refractometric detection |
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Authors: | Bengt Wittgren Bedich Porsch |
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Affiliation: | a Substance Analysis, Analytical Development, PAR&D, AstraZeneca R&D Mölndal, SE-431 83, Molndal, Sweden b Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06, Prague, Czech Republic |
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Abstract: | Physico-chemical properties of cellulose derivatives are of considerable interest in many technical applications, for example, in the food and drug industry. Efficient and careful characterisation of these properties is thus highly desirable. In this study, two different size exclusion chromatography (SEC) systems, connected on-line either to a low-angle laser light scattering detector (LALLS) or to a multi-angle laser light scattering detector (MALLS), are employed for size characterisation of three batches of hydroxypropyl cellulose (HPC) from different manufacturers. All three samples turned out to have a weight average molar mass around 100,000 g/mol, but considerable differences concerning conformational properties were found. Two of the samples contained compact components, presumably aggregates of HPC, which were clearly detected by both SEC-systems. The third sample, obtained from another manufacturer, did not show any indication of aggregation. Both SEC-MALLS and SEC-LALLS are proven to be efficient techniques for characterisation of complex polysaccharides like HPC containing mixtures of solvated polymer chains, as well as micelle-like aggregates. |
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Keywords: | Hydroxypropyl cellulose Size exclusion chromatography Light scattering detection Molar mass distribution |
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